Most existing anticancer drugs are small, very potent molecules; their effectiveness is limited by their systemic toxicity, their narrow therapeutic window, their low drug loading, their size control, their scale-up, their formulation cost, but also by drug resistance and their limited cellular entry. In recent years, carbon nanotubes have been considered as a promising carrier for many drugs, including anticancer agents, due to their high specific surface area and effective targeting capabilities. The present work aims to study the potential of multi-walled carbon nanotubes (MWCNT) as a vector to target 6 mercaptopurine to cancerous tissues. MWCNTs were functionalized with carboxy groups and then loaded with 6 mercaptopurine (6MP) using the melting method to produce 6MP-loaded CNTs. The conjugate was characterized for drug loading efficiency, in vitro drug release, and release kinetics. The result indicates that a maximum of approximately 65% ​​trapping was achieved. The charged nanotubes were shown to release the drug for more than 20 hours and thus controlled the release. The release was found to follow the Zero Order and Hixson Crowell release model. Our work established a novel, easy-to-prepare formulation of MWCNTs with improved drug loading efficiency and increased dispersibility of CNTs and thus bioavailability at the cancer site with reduced systemic toxicity. Key words: Multi-walled carbon nanotubes, 6-mercaptopurine, anticancer, fusion method. Cancer is among the three leading causes of death in modern society, after heart and cerebrovascular diseases. Treating cancer has always been a challenge because anticancer chemotherapeutic agents are cytotoxic and cannot differentiate cancer cells from normal cells. This leads to the destruction or alteration of vi...... middle of paper ......ents like PEG to achieve a long half-life in the bloodstream, helping to prevent opsonization in vivo and reduce the uptake of the reticuloendothelial system[ 12]. In addition, many oxygen-containing groups, mainly carboxyl and hydroxyl, decorate the surface of CNTs oxidized by strong acids[13]. Several targeted anticancer delivery systems containing 6-mercaptopurine (6MP) have been reported[14–18]. . In the present study, 6MP (6,7-dihydro-3H-purine-6-thione)-loaded CNTs (Fig. 1) were grown by a slight modification of the fusion method [19]. Noncovalent functionalization of multi-walled carbon nanotubes (MWCNTs) was carried out using base treatment followed by HCl treatment. The functionalized MWCNTs were attached with 6MP by sonication in an appropriate medium (pH 7.4). The formulations were characterized for drug entrapment, in vitro release and release kinetics...