Definition: Biosafety level presents different types of biocontainment safety measures that are used to separate dangerous biological microbes in laboratories. There are different biosafety levels ranging from Biosafety Level 1 (BSL-1) to Biosafety Level 4 (BSL-4) and these are categories from lowest to highest respectively. Introduction and Background Biosafety has implemented different sets of controlled principles, activities, advanced techniques and organized practices that are used to prevent unintended and unintentional exposure to hazardous agents, i.e. pathogens and toxic substances, as well as their accidental release/release. The term “biosecurity” refers to the protection provided to valuable biological materials by taking into account appropriate control and accountability to eliminate and restrict their unauthorized release, diversion, access and misuse. There are many unique and distinctive issues that can be found in the animal rooms during animal activities. These pet stores are better than microbiology laboratories because, due to the nature of animal activities, they may present unique hazards, i.e. pathogens and toxic agents that have not been found in standard microbiology laboratories. Hazardous agents via aerosols can be generated by animals, including animal bites and scratches. Sometimes other zoonotic agents of toxic agents can become infected. Animal Biosafety Levels S. No Animal Biosafety Level (ABSL) Practices Primary Barriers (Safety Equipment) Facilities (Secondary Barriers) 1  It includes organisms that are not dangerous and cause infectious diseases. They have a minimum of potential risks to personnel and the environment.  It involves standard microbiological practices  No primary barrier  Eye and face protection mask, coats and gloves required  Bench/tool ​​and sink required2  It includes advanced practices, procedures and equipment as well as organized monitoring with the installation of ABSL-1. This level includes moderate potential hazardous agents that pose a moderate risk to personnel and a low risk to the community.  This involves standard microbiological practices as well as limited access  Universal biohazard warning sign  Instant precautions  Medical surveillance policies  Primary barriers  Physical containment Devices used for handling agents that cause infectious materials, i.e. through air and splashes  Required eye and face mask, coats and gloves Requires biosafety cabinet ABSL-1 and availability of autoclaves3  Involves infectious microbes both for personnel and the environment. They pose a high risk to personnel and a moderate risk to the environment. It includes advanced practices, procedures and equipment as well as organized control with the installation of ABSL-2. They can cause life-threatening illness through inhalation at the root of exposure.  It includes ABSL-2 practices and controlled access Decontamination of all contaminated waste